The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Prss23 exon 2 contains the translation initiation codon.
Exon 2 has been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 2 should result in loss of function of the Prss23 gene by deleting the entire codon region.
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2006.
Gene Family: Protease
Genetic Background: C57BL/6
Gene Symbol: Prss23
Gene Accession Number: NM_029614
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: protease, serine, 23