-
The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
-
Prkag2 exon 1 contains the translation initiation site.
-
The R528G point mutation has been introduced into the Prkag2 exon 15.
-
The positive selection marker (Puromycin resistance - PuroR) has been flanked by F3 sites and inserted in intron 14.
-
The constitutive PM allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
-
The constitutive PM line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
-
Remaining recombination site will be located in a non-conserved region of the genome.
-
Datamining and Design performed in 2010.
Gene Family:
Kinase Genetic Background:
C57BL/6 Gene Symbol:
Prkag2 Gene Accession Number:
NM_001170555 Allele Type:
PM Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
protein kinase, AMP-activated, gamma 2 non-catalytic subunit