The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 1.2 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exon 3 should result in loss of function of the Prkag2 gene by generating a frameshift.
Datamining and Design performed in 2008.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Prkag2
Gene Accession Number: NM_145401
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit