The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 3 to 12 have been flanked by loxP sites (size of loxP-flanked region: 4.6 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 3 to 12 should result in loss of function of the Prkag1 gene.
According to NCBI and Ensembl databases, the Ddn gene (Entrez ID:13199, ENSMUSG00000059213) is located downstream of Prkag1 in a head-to-tail orientation.
Datamining and Design performed in 2008.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Prkag1
Gene Accession Number: NM_016781.2
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: protein kinase, AMP-activated, gamma 1 non-catalytic subunit