The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
The Prkaa2 exon 1 contains the translation initiation site.
The T172D point mutation has been introduced into Prkaa2 exon 5.
The positive selection marker (Puromycin resistance - PuroR) has been flanked by F3 sites and introduced into intron 5.
The constitutive PM allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The constitutive PM line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The remaining FRT recombination site will be located in a non-conserved region of the genome.
Datamining and Design performed in 2010.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Prkaa2
Gene Accession Number: NM_178143
Allele Type: PM
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: protein kinase, AMP-activated, alpha 2 catalytic subunit