The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exon 2 has been flanked by loxP sites (size of loxP-flanked region: 4.8 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exon 2 should result in loss of function of the Ppp1r3b gene by deleting the complete open reading frame.
The remaining recombination sites are located in non-conserved regions of the genome.
The corresponding conditional KO allele is available as line 10482.
Datamining and Design performed in 2009.
Gene Family: Phosphatase
Genetic Background: C57BL/6
Gene Symbol: Ppp1r3b
Gene Accession Number: NM_177741.3
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: protein phosphatase 1, regulatory (inhibitor) subunit 3B