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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Pkn3 exon 1 contains the translation initiation codon.
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Exons 2-6 have been flanked by loxP sites.
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Deletion of exons 2-6 of the Pkn3 gene should result in loss of function by generating a frameshift.
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The remaining recombination sites are located in non-conserved regions of the genome.
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The corresponding conditional allele is available as line 8077.
- Datamining and Design performed in 2006.
Gene Family:
Kinase Genetic Background:
C57BL/6 Gene Symbol:
Pkn3 Gene Accession Number:
NM_153805 Allele Type:
KO Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
protein kinase N3
