The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Pkn3 exon 1 contains the translation initiation codon.
Exons 2-6 have been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exons 2-6 of the Pkn3 gene should result in loss of function by generating a frameshift.
The remaining recombination sites are located in non-conserved regions of the genome.
The corresponding conditional allele is available as line 8077.
Datamining and Design performed in 2006.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Pkn3
Gene Accession Number: NM_153805
Allele Type: KO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: protein kinase N3