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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
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Pkmyt1 exon 2 contains the translation initiation codon.
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Exon 3 has been flanked by loxP sites.
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
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Deletion of exon3 should result in loss of function of the Pkmyt1 gene by generating a frameshift (premature stop codon in exon 4).
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The remaining recombination sites are located in intron regions of the genome.
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Data mining and Design performed in 2005.
Gene Family:
Kinase Genetic Background:
C57BL/6 Gene Symbol:
Pkmyt Gene Accession Number:
NM_023058 Allele Type:
cKO Source:
Mouse Clinical Institute (MCI) Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
protein kinase, membrane associated tyrosine/threonine 1
