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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
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P2ry14 exon 3 contains the translation initiation codon.
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Exons 2-3 have been flanked by loxP sites.
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Deletion of exons 2-3 should result in loss of function of the P2ry14 gene by deleting the G protein-coupled receptor domain.
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The remaining recombination sites are located in intron regions of the genome.
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Data mining and Design performed in 2004.
Gene Family:
G-protein coupled receptor Genetic Background:
C57BL/6 Gene Symbol:
P2ry14 Gene Accession Number:
NM_001008497 Allele Type:
KO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
purinergic receptor P2Y, G-protein coupled, 14
