The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
P2ry14 exon 3 contains the translation initiation codon.
Exons 2-3 have been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exons 2-3 should result in loss of function of the P2ry14 gene by deleting the G protein-coupled receptor domain.
The remaining recombination sites are located in intron regions of the genome.
Data mining and Design performed in 2004.
Gene Family: G-protein coupled receptor
Genetic Background: C57BL/6
Gene Symbol: P2ry14
Gene Accession Number: NM_001008497
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: purinergic receptor P2Y, G-protein coupled, 14