The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 2 to 4 have been flanked by loxP sites (size of loxP-flanked region: 3.5 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 2-4 should result in loss of function of the Ormdl3 gene, by removing the complete coding sequence.
Datamining and Design performed in 2008.
Gene Family: Glycoprotein
Genetic Background: C57BL/6
Gene Symbol: Ormdl3
Gene Accession Number: NM_025661.2
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: ORM1-like 3 (S. cerevisiae)