The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 3 to 7 have been flanked by loxP sites (size of loxP-flanked region: 2.2 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 3 to 7 should result in loss of function of the Nox1 gene.
The corresponding constitutive KO allele is available as line 9255.
Datamining and Design performed in 2008.
Gene Family: Oxidase
Genetic Background: C57BL/6
Gene Symbol: Nox1
Gene Accession Number: NM_172203.1
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: NAPDH oxidase 1