Nod2 - Model 12910 - cTTG

• The targeting vector has been transfected into TaconicArtemis C57Bl/6 ES cell line.
• The following elements have been inserted into the ROSA26 locus:


• A CAG promoter cassette.
• A loxP-flanked transcription termination cassette (STOP) containing a combination of polyadenylation signals.
• The Nod2 ORF together with a Kozak sequence (GCCACC).
• The hGH polyadenylation signal and an additional polyadenylation signal.


• The conditional TTG line has been generated by crossing chimeras to C57BL/6NTac animals.
• The constitutive TTG allele can be generated by Cre-mediated recombination by crossing line 12910 to a Cre-Deleter.
• Deletion of the hGHpA cassette should result in transcription of the Nod2 cDNA.
• The presence of the NeoR cassette will terminate the transcription from the ROSA26 promoter.
• Datamining and Design performed in 2012.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

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