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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exon 2 has been flanked by loxP sites (size of loxP-flanked region: 1.4 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 2 should result in loss of function of the Mylip gene by deleting part of the FERM domain and by generating a frameshift from exon 1 to exons 3 to 6 (premature Stop codon in exon 3).
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The remaining recombination sites are located in non-conserved regions of the genome.
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The corresponding constitutive KO allele is available as line 10865.
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Datamining and Design performed in 2009.
Gene Family:
Ligase Genetic Background:
C57BL/6 Gene Symbol:
Mylip Gene Accession Number:
NM_153789.3 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
myosin regulatory light chain interacting protein