The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 2.7 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 3 should result in loss of function of the Mstn gene, by removing the exon encoding the TGFb domain.
Datamining and Design performed in 2008.
Gene Family: Growth factor
Genetic Background: C57BL/6
Gene Symbol: Mstn
Gene Accession Number: NM_010834.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: myostatin