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Mmab - Model 9984 - KO

Constitutive Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 9984
    C57BL/6-Mmabtm1.2 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 1 contains the translation initiation codon.
  • Exons 5 to 7 have been flanked by loxP sites (size of loxP-flanked region: 1.5 kb).
  • The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
  • The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
  • Deletion of exons 5-7 should result in loss of function of the Mmab gene by deleting half of the catalytic domain and by generating a frameshift to all downstream exons.
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • According to NCBI and Ensembl databases, the Mvk gene (NCBI ID: 17855, Ensembl: ENSMUSG00000041939) is located approx. 7 kb upstream of Mmab exon 5 in a head-to-head orientation.
  • The corresponding conditional KO allele is available as line 10174.
  • Datamining and Design performed in 2008.

Gene Family:

Transferase

Genetic Background:

C57BL/6

Gene Symbol:

Mmab

Gene Accession Number:

NM_029956.3

Allele Type:

KO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

methylmalonic aciduria (cobalamin deficiency) type B homolog (human)

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.