The targeting vector has been generated using C57BL/6J DNA and transfected into 129Sv/Pas ES cell line.
Mlycd exon 1 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 3 should result in loss of function of the Mlycd gene by generating a frameshift (premature stop codon in exon 4).
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2004.
Gene Family: Decarboxylase
Genetic Background: C57BL/6; 129Sv/Pas
Gene Symbol: Mlycd
Gene Accession Number: NM_019966
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: malonyl-CoA decarboxylase