Mir122a - Model 9568 - cTTG

This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 9568
    C57BL/6-Gt(ROSA)26Sortm1 (CAG-Mir122a)Mrl
  • The targeting vector has been transfected into TaconicArtemis C57Bl/6 ES cell line.
  • The following elements have been inserted into the ROSA26 locus: a CAG promoter cassette; a loxP-flanked transcription termination cassette (STOP) containing a combination of polyadenylation signals; the miR30-miR122a transgene and a polyadenylation site (hGH polyA).
  • The conditional TTG line has been generated by crossing chimeras to C57BL/6NTac animals.
  • The constitutive TTG allele can be generated by Cre-mediated recombination by crossing the line to a Cre-Deleter.
  • Deletion of the STOP cassette should result in transcription of the mir122a transgene.
  • The presence of the NeoR cassette will terminate the transcription from the ROSA26 promoter.
  • Datamining and Design performed in 2008.

Gene Family:

microRNA

Gene Symbol:

Mir122a

Gene Accession Number:

NR_029600

Allele Type:

cTTG

Source:

TaconicArtemis

Species:

Mouse

Official Gene Name:

microRNA 122a

GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

For Profit Pricing:

Price per Line ($)
$31,500

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€28,650

Nonprofit Pricing:

Price per Line (€)
€5,005
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