Mir122a - Model 9568 - cTTG

• The targeting vector has been transfected into TaconicArtemis C57Bl/6 ES cell line.
• The following elements have been inserted into the ROSA26 locus: a CAG promoter cassette; a loxP-flanked transcription termination cassette (STOP) containing a combination of polyadenylation signals; the miR30-miR122a transgene and a polyadenylation site (hGH polyA).
• The conditional TTG line has been generated by crossing chimeras to C57BL/6NTac animals.
• The constitutive TTG allele can be generated by Cre-mediated recombination by crossing the line to a Cre-Deleter.
• Deletion of the STOP cassette should result in transcription of the mir122a transgene.
• The presence of the NeoR cassette will terminate the transcription from the ROSA26 promoter.
• Datamining and Design performed in 2008.

GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

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