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Mark4 - Model 8070 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 8070
    C57BL/6-Mark4tm1.1Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
  • Mark4 exon 1 contains the translation initiation codon.
  • Exons 2-3 have been flanked by loxP sites.
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination. Deletions of exons 2-3 should result in loss of function of the Mark4 gene by deleting the catalytic domain and generating a frameshift (premature stop codon in exon 4).
  • The remaining recombination sites are located in intron regions of the genome.
  • Datamining and Design performed in 2004.

Gene Family:

Kinase

Genetic Background:

C57BL/6

Gene Symbol:

Mark4

Gene Accession Number:

NM_172279

Allele Type:

KO

Source:

Institut Clinique de la Souris (ICS), Illkirch, France

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

MAP/microtubule affinity-regulating kinase 4

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

For-Profit Pricing:

Price per Line ($)
$30,000

Non-Profit Pricing:

Price per Line ($)
$5,500

For-Profit Pricing:

Price per Line (€)
€27,300

Non-Profit Pricing:

Price per Line (€)
€5,005