The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6J ES cell line.
Mark3 exon 1 contains the translation initiation codon.
Exons 8-10 have been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletions of exons 8-10 should result in loss of function of the Mark3 gene by generating a frameshift (premature stop codon in exon 11).
The remaining recombination sites are located in intron regions of the genome.
The corresponding constitutive KO allele is available as line 8072.
Datamining and Design performed in 2005.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Mark3
Gene Accession Number: NM_021516
Allele Type: cKO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: MAP/microtubule affinity-regulating kinase 3