The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Mapt exon 2 contains the translation initiation codon.
Exon 5 has been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 5 should result in loss of function of the Mapt gene by deleting the Microtubule associated protein domain.
The remaining recombination sites are located in intron regions of the genome.
The conditional KO allele is also available as line 8977.
Data mining and Design performed in 2007.
Gene Family: Microtubule
Genetic Background: C57BL/6
Gene Symbol: Mapt
Gene Accession Number: NM_010838
Allele Type: KO
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: microtubule-associated protein tau