The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon 1 and approx. 1.5 kb of sequence upstream of exon 1 (promoter region) has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 1 (including part of the proximal promoter and 3´ UTR) will result in loss of function of the Mafb gene by deleting the complete gene.
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2009.
Gene Family: Transcription factor
Genetic Background: C57BL/6
Gene Symbol: Mafb
Gene Accession Number: NM_010658.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian)