Lpin3 - Model 10584 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 10584
    C57BL/6-Lpin3tm1.1 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 2 contains the translation initiation codon.
  • Exons 4 to 7 have been flanked by loxP sites (size of loxP-flanked region: 2.9 kb).
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 4 to 7 should result in loss of function of the Lpin3 gene by deleting the C-terminal part of the Lipin domain and the potential nuclear localization signal and by generating a frameshift from exon 3 to exons 8-11 (premature Stop codon in exon 8).
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • According to NCBI and Ensembl databases, there are two genes in the neighborhood of the Lpin3 gene:

    • The Zhx3 gene (Entrez ID: 320799; ENSMUSG00000035877) is located approx. 22 kb upstream of Lpin3 exon 4 in a head-to-head orientation.
    • The Emilin3 gene (Entrez ID: 280635; ENSMUSG00000050700) is located approx. 9 kb downstream of Lpin3 exon 7 in a tail-to-tail orientation.

  • The corresponding constitutive KO allele is available as line 10615.
  • Datamining and Design performed in 2009.

Gene Family:

Phosphatase

Genetic Background:

C57BL/6

Gene Symbol:

Lpin3

Gene Accession Number:

NM_022883.2

Allele Type:

cKO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
The KO Repository lines exist as cryopreserved materials. Cohorts can typically be produced and delivered to the customer within 14-16 weeks of order receipt. For most lines, the mouse ES cells used in generating the model are of the 129S5 strain. Lines are stored primarily as cryopreserved sperm on a mixed 129S5 x C57BL/6J-Tyrc-Brd background. Lines are recovered using cryopreserved sperm and C57BL/6NTac oocyte donors. Some lines are stored as cryopreserved embryos and when revitalized will result in a 129/S5 x C57BL/6J-Tyrc-Brd background.

For standard orders clients will receive:

  • A minimum of four (4) mutant mice, heterozygous for the selected mutation on a mixed 129S5;B6 background.
  • Validated genotyping protocols
  • Animal health data
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
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