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Lpin3 - Model 10584 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 10584
    C57BL/6-Lpin3tm1.1 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 2 contains the translation initiation codon.
  • Exons 4 to 7 have been flanked by loxP sites (size of loxP-flanked region: 2.9 kb).
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 4 to 7 should result in loss of function of the Lpin3 gene by deleting the C-terminal part of the Lipin domain and the potential nuclear localization signal and by generating a frameshift from exon 3 to exons 8-11 (premature Stop codon in exon 8).
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • According to NCBI and Ensembl databases, there are two genes in the neighborhood of the Lpin3 gene:

    • The Zhx3 gene (Entrez ID: 320799; ENSMUSG00000035877) is located approx. 22 kb upstream of Lpin3 exon 4 in a head-to-head orientation.
    • The Emilin3 gene (Entrez ID: 280635; ENSMUSG00000050700) is located approx. 9 kb downstream of Lpin3 exon 7 in a tail-to-tail orientation.

  • The corresponding constitutive KO allele is available as line 10615.
  • Datamining and Design performed in 2009.

Gene Family:

Phosphatase

Genetic Background:

C57BL/6

Gene Symbol:

Lpin3

Gene Accession Number:

NM_022883.2

Allele Type:

cKO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

Taconic continues to strive to gain full access to all models for both non-profit and for-profit organizations. However, at this time some models are currently not available to commercial institutions. Please be sure to check back often as more models are made available on a routine basis.

Non-profit Pricing:

Price per Line ($)
$5,500

Non-profit Pricing:

Price per Line (€)
€5,005
The KO Repository lines exist as cryopreserved materials. Cohorts can typically be produced and delivered to the customer within 14-16 weeks of order receipt. For most lines, the mouse ES cells used in generating the model are of the 129S5 strain. Lines are stored primarily as cryopreserved sperm on a mixed 129S5 x C57BL/6J-Tyrc-Brd background. Lines are recovered using cryopreserved sperm and C57BL/6NTac oocyte donors. Some lines are stored as cryopreserved embryos and when revitalized will result in a 129/S5 x C57BL/6J-Tyrc-Brd background.

For standard orders clients will receive:

  • A minimum of four (4) mutant mice, heterozygous for the selected mutation on a mixed 129S5;B6 background.
  • Validated genotyping protocols
  • Animal health data
Nonprofit users: Nonprofit users must execute an MTA prior to accessing a GEM Collection Model.
Nonprofit order process is as follows:
  1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
  2. Nonprofit Investigator fills out MTA (including scientific workplan answering 4 basic questions) and Investigator's institution reviews, signs and sends to info@taconic.com.
  3. MTA is executed by all parties and release of model is approved.
  4. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery and shipment costs if this has not already been provided.

Taconic begins cryorecovery upon receipt of PO and delivers model to requestor. It is the requestor's obligation to determine that the GEM meets their research needs.

Access to this model for nonprofit customers requires approval of a third-party Material Transfer Agreement (MTA). The terms of this MTA are non-negotiable. MTA approval may require 4-6 months due to circumstances outside our control. Once approved and the purchase confirmed, order fulfillment will require an additional 14-16 weeks. If you wish to continue your interest in this model, please complete the GEM Collection Inquiry Form. In addition, Taconic also offers complete model generation services that may allow for a reduced timeline to delivery of a comparable model. If you are interested in learning more, please request a GEMs Design Consultation.