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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 2 to 7 have been flanked by loxP sites (size of loxP-flanked region: 2.7 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 2 to 7 should result in loss of function of the Lpcat4 gene by deleting both transmembrane domains and part of the catalytic domain and by generating a frameshift from exon 1 to exon 8 (premature Stop codon in exon 9).
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The remaining recombination sites are located in non-conserved regions of the genome.
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According to NCBI and Ensembl databases, the Nut gene (Entrez ID: 213765; ENSMUSG00000041358) is located approx. 4.5 kb downstream of Lpcat4 exon 7 in a tail-to-tail orientation.
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The corresponding constitutive KO allele is available as line 10815.
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Datamining and Design performed in 2009.
Gene Family:
Transferase Genetic Background:
C57BL/6 Gene Accession Number:
NM_207206.1 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
lysophosphatidylcholine acyltransferase 4
