Lpcat3 - Model 11200 - cKO

• Targeting strategy allows generation of conditional and constitutive Knockout Lpcat3 alleles.
• Lpcat3 exon 1 contains the translation initiation site.
• Exon 3 has been flanked by loxP sites (loxP-flanked region: 0.7 kb).
• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
• Exon 1 contains the translation initiation codon.
• Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 0.7 kb).
• The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 3 should result in loss of function of the Lpcat3 gene by deleting two transmembrane domains (SwissProt #Q91V01) and by generating a frameshift from exon 2 to exon 4 (premature Stop codon in exon 4).
• The remaining recombination sites are located in non-conserved regions of the genome.
• According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Lpcat3 gene:
  
  
  • The Emg1 gene (Entrez ID: 14791; ENSMUSG00000004268) is located approx. 5.0 kb downstream of Lpcat3 exon3 in a tail-to-tail orientation.
  • The Phb2 gene (Entrez ID: 12034; ENSMUSG00000004264) is located approx. 13.0 kb downstream of Lpcat3 exon3 in a tail-to-head orientation.
• The corresponding constitutive KO allele is available as line 10932.
• Datamining and Design performed in 2009.

NOTICE: Access to this model is conditioned on the requestor's institution agreeing to the terms and conditions of a Material Transfer Agreement (MTA).

Steps to initiate and place order:

1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
2. Nonprofit Investigator inquires on Taconic website.
3. Taconic will answer questions about the GEM lines and the process.
4. Taconic sends request for MTA.
5. Nonprofit Investigator provides scientific workplan answering 4 basic questions.
6. MTA is issued for Nonprofit Investigator/institution review and signature.
7. MTA is executed by all parties and release of GEM is approved.
8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and shipment costs.
9. Taconic begins cryorecovery upon receipt of PO and delivers GEM line to requestor (See pricing and Deliverables).

It is the requestor's obligation to determine that the GEM meets their research needs.

Learn more about Terms of Use, The Scientific Workplan and Frequently Asked Questions.

Upon execution of the MTA terms of use, requestor needs to provide purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and additional shipment costs.

1. Taconic will begin standard derivation of cryopreserved material (sperm or embryos) upon receipt of PO from recipient.
2. Taconic will conduct testing using its International Health Monitoring System™ (IHMS™) to ensure that the mice meet a Murine Pathogen Free™ (MPF™) health status.
3. Taconic will genotype the recovered mice prior to shipment.
4. Taconic will provide a health status report, the genotyping results of the recovered animals and genotyping assay information for the requested GEM line.
5. Taconic shall deliver a minimum of two (2) mice heterozygous at the selected gene of interest of a requested GEM line.
6. Taconic will make shipping arrangements for the requested GEM to the Licensed Nonprofit recipient.
7. Nonprofit investigator is responsible for covering the shipment costs.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.