The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
The Lnpep exon 1 contains the translation initiation codon.
The H464Y and H468Y point mutations have been introduced into Lnpep exon 6 (TKIIAHELAHQWFGN to TKIIAYELAYQWFGN)
A positive selection marker (Puromycin resistance) has been flanked by F3 sites and introduced into intron 6
The constitutive PM allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The constitutive PM line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The remaining FRT recombination site will be located in a non-conserved region of the genome.
Datamining and Design performed in 2009.
Gene Family: Peptidase
Genetic Background: C57BL/6
Gene Symbol: Lnpep
Gene Accession Number: NM_172827
Allele Type: PM
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: leucyl/cystinyl aminopeptidase