The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 1.5 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 3 should result in loss of function of the Lclat1 gene by deleting part of the catalytic domain and by generating a frameshift from exon 2 to all downstream exons (premature Stop codon in exon 4).
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2009.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Lclat1
Gene Accession Number: NM_001081071.1
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: lysocardiolipin acyltransferase 1