-
The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
-
Exon 1 contains the translation initiation codon.
-
Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 1.1 kb).
-
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
-
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
-
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 3 should result in loss of function of the Kctd10 gene by deleting part of the BTB/POZ-like domain and by generating a frameshift to all downstream exons.
-
According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Kctd10 gene:
- The Ube3b gene (NCBI ID: 117146, Ensembl: ENSMUSG00000029577) is located approx. 10 kb upstream of Kctd10 exon 3 in a head-to-head orientation.
- The Myo1h gene (NCBI ID: 231646, Ensembl: ENSMUSG00000066952) is located approx. 6.5 kb downstream of Kctd10 in a tail-to-tail orientation.
-
The corresponding constitutive KO allele is available as line 9991.
-
Datamining and Design performed in 2008.
Gene Family:
Phosphoprotein Genetic Background:
C57BL/6 Gene Symbol:
Kctd10 Gene Accession Number:
NM_001159941 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
potassium channel tetramerisation domain containing 10
