Kcnj1 - Model 11481 - cKO

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
• Kcnj1 exon 2 contains the complete open reading frame (ORF).
• Exon 2 has been flanked by loxP sites (size of loxP-flanked region 3.5 kb).
• The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• Deletion of exon 2 should result in loss of function of the Kcnj1 gene by deleting the complete ORF and by removing the 3'UTR.
• The remaining recombination sites are located in non-conserved regions of the genome.
• The corresponding constitutive KO allele is available as line 12079.
• Datamining and Design performed in 2010.

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.