The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 0.9 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 3 should result in the loss of function of the Jak1 gene by deleting part of the FERM domain and by generating a frameshift from exon 2 to exons 4 and 5 (premature Stop codon in exon 4).
The remaining recombination sites are located in non-conserved regions of the genome.
The corresponding constitutive KO allele is available as line 12347.
Datamining and Design performed in 2012.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Jak1
Gene Accession Number: NM_146145.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: Janus kinase 1