Irf5 - Model 12685 - KO

Constitutive Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 12685
    C57BL/6-Irf5 tm1.2 Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
  • Irf5 exon 1 contains the translation initiation codon.
  • Exons 3 to 9 have been flanked by loxP sites (size of loxP-flanked region 4.2 kb).
  • A polyadenylation signal (hGHpA: human Growth Hormone polyadenylation signal) has been inserted 3' of the Irf5 gene in order to prevent downstream transcription in the constitutive KO allele.
  • The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
  • The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
  • Deletion of exons 3 to 9 should result in loss of function of the Irf5 gene by removing most of the gene. After Cre-mediated deletion of exons 3 to 9, the hGHpA cassette should prevent downstream transcription.
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • The corresponding conditional KO allele is available as line 12646.
  • Datamining and Design performed in 2012.
  • Gene Family:

    Regulatory Factor

    Genetic Background:


    Gene Symbol:


    Gene Accession Number:


    Allele Type:





    Cryopreserved at Taconic



    Official Gene Name:

    interferon regulatory factor 5

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

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