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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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The Irak4 exon 2 contains the translation initiation codon.
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The K213A point mutation has been introduced into the Irak4 exon5.
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The positive selection marker (PuroR) has been flanked by F3 sites and inserted into intron 5.
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The constitutive PM allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The constitutive PM line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The remaining F3 recombination site will be located in a non-conserved region of the genome.
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Datamining and Design performed in 2010.
Gene Family:
Kinase Genetic Background:
C57BL/6 Gene Symbol:
Irka4 Gene Accession Number:
NM_029926 Allele Type:
PM Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
interleukin-1 receptor-associated kinase 4