The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
The Irak4 exon 2 contains the translation initiation codon.
The K213A point mutation has been introduced into the Irak4 exon5.
The positive selection marker (PuroR) has been flanked by F3 sites and inserted into intron 5.
The constitutive PM allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The constitutive PM line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The remaining F3 recombination site will be located in a non-conserved region of the genome.
Datamining and Design performed in 2010.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Irka4
Gene Accession Number: NM_029926
Allele Type: PM
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: interleukin-1 receptor-associated kinase 4