Il13ra1 - Model 7895 - KO

Constitutive Knock Out

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

  • Model #
  • Genotype
  • Nomenclature
  • 7895
    B6;129S2-IL13ra1tm1.2mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into 129Sv/Pas ES cell line.
  • Il13ra1 exon 1 contains the translation initiation codon.
  • Exon 3 has been flanked by loxP sites.
  • The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
  • The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
  • Deletion of exon 3 should result in loss of function of the Il13ra1 gene by generating a frame shift (premature stop codon in exon 4).
  • The remaining recombination sites are located in intron regions of the genome.
  • The constitutive KO line is also available in a congenic C57BL/6 genetic background, line 8031.
  • Datamining and Design performed in 2005.

Gene Family:

Receptor

Genetic Background:

C57BL/6; 129Sv/Pas

Gene Symbol:

Il13ra1

Gene Accession Number:

NM_133990

Allele Type:

KO

Source:

Institut Clinique de la Souris (ICS), Illkirch, France

Availability:

Cryopreserved at Taconic

Species:

Mouse

The KO Repository lines exist as cryopreserved materials. Cohorts can typically be produced and delivered to the customer within 14-16 weeks of order receipt. For most lines, the mouse ES cells used in generating the model are of the 129S5 strain. Lines are stored primarily as cryopreserved sperm on a mixed 129S5 x C57BL/6J-Tyrc-Brd background. Lines are recovered using cryopreserved sperm and C57BL/6NTac oocyte donors. Some lines are stored as cryopreserved embryos and when revitalized will result in a 129/S5 x C57BL/6J-Tyrc-Brd background.

For standard orders clients will receive:

  • A minimum of four (4) Mutant Mice, heterozygous for the Selected Mutation, including at least one (1) breeding pair on a mixed 129S5;B6 background.
  • Validated genotyping protocols
  • Animal health data

Commercial Pricing:

Number of Lines PurchasedPrice per Line ($)Price per Line for Comprehensive Phenotype Data ($)
Single Line $45,000 $25,000
2 Lines $40,500 $25,000
3 Lines $39,375 $25,000
4+ Lines $38,250 $25,000

Nonprofit Pricing:

Price per Line ($)Price per Line for Comprehensive Phenotype Data ($)
$30,000 $15,000
Basic Phenotype Package: $5,000
Multi-line discounts will be applied to lines ordered on a single purchase order (PO).

Commercial Pricing:

Number of Lines PurchasedPrice per Line (€)Price per Line for Comprehensive Phenotype Data (€)
Single Line €42,750 €23,750
2 Lines €38,475 €23,750
3 Lines €37,406 €23,750
4+ Lines €36,338 €23,750

Nonprofit Pricing:

Price per Line (€)Price per Line for Comprehensive Phenotype Data (€)
€28,500 €14,250
Basic Phenotype Package: €4,750
Multi-line discounts will be applied to lines ordered on a single purchase order (PO).