The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
Htr7 exon 1 was disrupted by the neomycin resistance cassette, which is flanked by loxP sites.
Chimeric mice were produced and germ line transmission achieved.
The neomycin resistance gene has not been deleted in the constitutive KO mice.
Disruption of exon 1 should result in loss of function of the Htr7 gene by deleting functional domains of the protein.
Htr7 constitutive KO mice are in a mixed C57BL/6-129SvEv genetic background, but backcrossed to C57BL/6 for 3 generations.
See M.R. Guscott et al, Neuropharmacology 44, 1031-1037, 2003, for further details.
Gene Family: Receptor
Genetic Background: C57BL/6;129SvEv
Gene Symbol: Htr7
Gene Accession Number: NM_008315
Allele Type: KO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: 5-hydroxytryptamine (serotonin) receptor 7