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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 4 contains the translation initiation codon.
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Exon 4 has been flanked by loxP sites (size of loxP-flanked region: 6.6 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 4 should result in loss of function of the Hivep3 gene, by removing the exon containing the translation initiation site and encoding most of the protein. The next in-frame ATG codons are located in exons 6 and 7.
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Datamining and Design performed in 2008.
Gene Family:
zinc finger protein Genetic Background:
C57BL/6 Gene Symbol:
Hivep3 Gene Accession Number:
NM_010657.3 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
human immunodeficiency virus type I enhancer binding protein 3
