The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 4 contains the translation initiation codon.
Exon 4 has been flanked by loxP sites (size of loxP-flanked region: 6.6 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 4 should result in loss of function of the Hivep3 gene, by removing the exon containing the translation initiation site and encoding most of the protein. The next in-frame ATG codons are located in exons 6 and 7.
Datamining and Design performed in 2008.
Gene Family: zinc finger protein
Genetic Background: C57BL/6
Gene Symbol: Hivep3
Gene Accession Number: NM_010657.3
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: human immunodeficiency virus type I enhancer binding protein 3