The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 5 and 6 have been flanked by loxP sites (size of loxP-flanked region: 2.2 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 5-6 should result in loss of function of the Hibadh gene by deleting part of the catalytic domain (including residue K208 described as the active site (SwissProt #Q99L13) and by generating a frameshift from exon 4 to exons 7 and 8 (premature Stop codon in exon 7).
Datamining and Design performed in 2009.
Gene Family: Dehydrogenase
Genetic Background: C57BL/6
Gene Symbol: Hibadh
Gene Accession Number: NM_145567.1
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: 3-hydroxyisobutyrate dehydrogenase