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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 5 and 6 have been flanked by loxP sites (size of loxP-flanked region: 2.2 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exons 5-6 should result in loss of function of the Hibadh gene by deleting part of the catalytic domain (including residue K208 described as the active site (SwissProt #Q99L13) and by generating a frameshift from exon 4 to exons 7 and 8 (premature Stop codon in exon 7).
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Datamining and Design performed in 2009.
Gene Family:
Dehydrogenase Genetic Background:
C57BL/6 Gene Symbol:
Hibadh Gene Accession Number:
NM_145567.1 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
3-hydroxyisobutyrate dehydrogenase
