-
The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
-
Hdac6 exon 2 contains the translation initiation codon.
-
Exons 11, 12, 13, 14 have been flanked by loxP sites.
-
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
-
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
-
Deletion of exons 11, 12, 13, 14 should result in loss of function of the Hdac6 gene by generating a frameshift (premature stop codon in exon 16).
-
The remaining recombination sites are located in intron regions of the genome.
-
The corresponding conditional KO allele is available as line 8049.
-
Datamining and Design performed in 2005.
Gene Family:
Deacetylase Genetic Background:
C57BL/6 Gene Symbol:
Hdac6 Gene Accession Number:
NM_010413 Allele Type:
KO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
histone deacetylase 6
