The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon3 has been flanked by loxP sites (size of loxP-flanked region: 0.5 kb).
- FRT-flanked neomycin cassette was removed by crossing with a Flp deleter mouse on the C57BL/6 background.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exon 3 should result in loss of function of the Hdac11 by generating a frameshift.
Datamining and Design performed in 2003.
Gene Family: Deacetylase
Genetic Background: C57BL/6
Gene Symbol: Hdac11
Gene Accession Number: NM_144919
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: histone deacetylase 11