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The targeting vector has been generated using C57BL/6 DNA and transfected into C57BL/6 ES cell line.
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Hcst exons 3 and 4 were removed by the neomycin resistance cassette, which is flanked by loxP sites.
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The neomycin resistance gene has been deleted by transient transfection of Cre in targeted ES cells.
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Chimeric mice were produced and germ line transmission achieved.
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Deletion of exons 3 and 4 should result in loss of function of the Hcst gene by deleting critical domains for the expression of a functional protein.
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The remaining recombination sites are located in intron regions of the genome.
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See Hyka-Nouspikel & Phillips, Immunological reviews 214, 2006, for further details.
Gene Family:
Transducer Genetic Background:
C57BL/6 Gene Symbol:
Hcst Gene Accession Number:
NM_011827 Allele Type:
KO Source:
DNAX Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
hematopoietic cell signal transducer