Hcst - Model 12667 - KO

• The targeting vector has been generated using C57BL/6 DNA and transfected into C57BL/6 ES cell line.
• Hcst exons 3 and 4 were removed by the neomycin resistance cassette, which is flanked by loxP sites.
• The neomycin resistance gene has been deleted by transient transfection of Cre in targeted ES cells.
• Chimeric mice were produced and germ line transmission achieved.
• Deletion of exons 3 and 4 should result in loss of function of the Hcst gene by deleting critical domains for the expression of a functional protein.
• The remaining recombination sites are located in intron regions of the genome.
• See Hyka-Nouspikel & Phillips, Immunological reviews 214, 2006, for further details.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

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