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Grn - Model 10150

Random Transgenic

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 10150
    C57BL/6-Tg(Grn)26 Mrl
  • A BAC containing the mouse Grn gene was engineered a ~30 kb DNA region encompassing the Grn promoter and exons 1-13, but not the neighboring Rundc3a, Slc25a39, and Itga2b genes.
  • The BAC construct was microinjected in C57BL6 mice by pronuclear injection.
  • Putative transgenic founders were identified by genotyping and bred for germ line transmission with C57BL/6 mice.
  • Heterozygous transgenic mice were identified by genotyping.
  • Line 10015, C57BL/6-Tg(Grn)42 Mrl, has the lowest expression level of the BAC transgene in the brain (2 fold at mRNA level).
  • Line 10016, C57BL/6-Tg(Grn)33 Mrl, has second highest expression level of the BAC transgene in the brain (5-7 fold at mRNA level).
  • Line 10150, C57BL/6-Tg(Grn)26 Mrl, has the highest expression level of the BAC transgene in the brain (10-14 fold at mRNA level).
  • Datamining and Design performed in 2008.

Gene Family:

Growth factor

Genetic Background:

C57BL/6

Gene Symbol:

Grn

Gene Accession Number:

NM_008175

Allele Type:

TG

Source:

Xenogen

Availability:

Cryopreserved at Taconic

Species:

Mouse

Taconic continues to strive to gain full access to all models for both non-profit and for-profit organizations. However, at this time some models are currently not available to commercial institutions. Please be sure to check back often as more models are made available on a routine basis.

Non-profit Pricing:

Price per Line ($)
$5,500

Non-profit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Nonprofit users: Nonprofit users must execute an MTA prior to accessing a GEM Collection Model.
Nonprofit order process is as follows:
  1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
  2. Nonprofit Investigator fills out MTA (including scientific workplan answering 4 basic questions) and Investigator's institution reviews, signs and sends to info@taconic.com.
  3. MTA is executed by all parties and release of model is approved.
  4. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery and shipment costs if this has not already been provided.

Taconic begins cryorecovery upon receipt of PO and delivers model to requestor. It is the requestor's obligation to determine that the GEM meets their research needs.

Access to this model for nonprofit customers requires approval of a third-party Material Transfer Agreement (MTA). The terms of this MTA are non-negotiable. MTA approval may require 4-6 months due to circumstances outside our control. Once approved and the purchase confirmed, order fulfillment will require an additional 14-16 weeks. If you wish to continue your interest in this model, please complete the GEM Collection Inquiry Form. In addition, Taconic also offers complete model generation services that may allow for a reduced timeline to delivery of a comparable model. If you are interested in learning more, please request a GEMs Design Consultation.