The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 7 to 8 have been flanked by loxP sites (size of loxP-flanked region: 2.7 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 7-8 should result in loss of function of the Gpt2 gene by deleting part of the catalytic domain (including residue K340 described as the catalytic active site (SwissProt #Q8BGT5)) and by generating a frameshift from exon 6 to all downstream exons (premature Stop codon in exon 9).
Datamining and Design performed in 2009.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Gpt2
Gene Accession Number: NM_173866.2
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: glutamic pyruvate transaminase (alanine aminotransferase) 2