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The transgenic construct contains 5´ a floxed neo cassette, the 8.5 kb mouse insulin promotor (MIP), the rabbit b-globulin intron, the human GPR6 cDNA, and the SV40 polyA signal.
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The transgenic construct was transfected into C57BL/6NTac ES cell line.
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Copy numbers of random integrators were determined by Southern blotting.
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The neomycin resistance cassette was removed by Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The transgenic founders were subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Line 11210, M160 clone 13, contains 2-3 copies of the transgene.
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Line 11211, M160 clone 27, contains 10-25 copies of the transgene.
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Datamining and Design performed in 2008.
Gene Family:
G-protein coupled receptor Genetic Background:
C57BL/6 Gene Symbol:
Gpr6 Gene Accession Number:
NM_199058 Allele Type:
TG Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
G protein-coupled receptor 6