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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 4 and 5 have been flanked by loxP sites (size of loxP-flanked region: 1.5 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exons 4 and 5 should result in loss of function of the Gpr179 gene by deleting part of transmembrane domains and by generating a frame shift from exon 3 to downstream exons (premature Stop codon in exon 6).
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Datamining and Design performed in 2009.
Gene Family:
G-protein coupled receptor Genetic Background:
C57BL/6 Gene Symbol:
Gpr179 Gene Accession Number:
NM_001081220.1 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
G protein-coupled receptor 179
