The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Gpr139 exon 1 contains the translation initiation codon.
Exon 2 has been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 2 should result in loss of function of the Gpr139 gene by deleting part of the functional domain.
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2004.
Gene Family: G-protein coupled receptor
Genetic Background: C57BL/6
Gene Symbol: Gpr139
Gene Accession Number: NM_001024138
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: G protein-coupled receptor 139