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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
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Gpr137b exon 1 contains the translation initiation codon.
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Exon 2 has been flanked by loxP sites.
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Deletion of exon 2 should result in loss of function of the Gpr137b gene by generating a frameshift (premature Stop codon in exon 3).
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The remaining recombination sites are located in intron regions of the genome.
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The corresponding conditional KO allele is available as line 9753.
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Datamining and Design performed in 2007.
Gene Family:
G-protein coupled receptor Genetic Background:
C57BL/6 Gene Symbol:
Gpr137b Gene Accession Number:
NM_031999 Allele Type:
KO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic Species:
Mouse
