The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Gpr137b exon 1 contains the translation initiation codon.
Exon 2 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 2 should result in loss of function of the Gpr137b gene by generating a frameshift (premature Stop codon in exon 3).
The remaining recombination sites are located in intron regions of the genome.
The corresponding constitutive KO allele is available as line 9371.
Datamining and Design performed in 2007.
Gene Family: G-protein coupled receptor
Genetic Background: C57BL/6
Gene Symbol: Gpr137b
Gene Accession Number: NM_007806
Allele Type: cKO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: G protein-coupled receptor 137B