The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
Gpr12 coding exon was disrupted by the neomycin resistance cassette, which is flanked by loxP sites.
Chimeric mice were produced and germ line transmission achieved.
The neomycin resistance gene has not been deleted in the constitutive KO mice.
Disruption of the coding sequence should result in loss of function of the Gpr12 gene by deleting functional domains of the protein.
Gpr12 constitutive KO mice are in a mixed C57BL/6-129SvEv genetic background.
Gene Family: G-protein coupled receptor
Genetic Background: C57BL/6
Gene Symbol: Gpr12
Gene Accession Number: NM_001010941
Allele Type: KO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic