-
The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
-
Exon 2 contains the translation initiation codon.
-
Exons 17 to 20 have been flanked by loxP sites (size of loxP-flanked region: 4.3 kb).
-
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
-
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
-
Deletion of exons 17-20 should result in loss of function of the Gpr116 gene by deleting the exons encoding the 7 transmembrane domains and by generating a frameshift from exon 16 to exon 21.
-
Datamining and Design performed in 2009.
Gene Family:
G-protein coupled receptor Genetic Background:
C57BL/6 Gene Symbol:
Gpr116 Gene Accession Number:
NM_001081178.1 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
G protein-coupled receptor 116
