The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 17 to 20 have been flanked by loxP sites (size of loxP-flanked region: 4.3 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 17-20 should result in loss of function of the Gpr116 gene by deleting the exons encoding the 7 transmembrane domains and by generating a frameshift from exon 16 to exon 21.
Datamining and Design performed in 2009.
Gene Family: G-protein coupled receptor
Genetic Background: C57BL/6
Gene Symbol: Gpr116
Gene Accession Number: NM_001081178.1
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: G protein-coupled receptor 116