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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exon 3 has been flanked by loxP sites (size of loxP-flanked region: 2.0 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 3 should result in loss of function of the Gpc5 gene by deleting part of the glypican domain and by generating a frameshift from exon 2 to exons 4-7 (premature Stop codon in exon 4).
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The remaining recombination sites are located in non-conserved regions of the genome.
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Datamining and Design performed in 2009.
Gene Family:
Receptor Genetic Background:
C57BL/6 Gene Symbol:
Gpc5 Gene Accession Number:
NM_175500.3 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
glypican 5