The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 9 and 10 have been flanked by loxP sites (size of loxP-flanked region: 1.5 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 9 and 10 should result in loss of function of the Gpam gene by deleting part of the acetyl-transferase domain and by generating a frameshift from exon 8 to exons 11 and 12 (premature Stop codon in exon 11).
The remaining recombination sites are located in non-conserved regions of the genome.
The corresponding conditional KO allele is available as line 11106.
Datamining and Design performed in 2009.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Gpam
Gene Accession Number: NM_008149.3
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: glycerol-3-phosphate acyltransferase, mitochondrial