The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 1-5 (complete gene) and 1333 bp of sequence upstream of exon 1 (promoter region) have been flanked by loxP sites (size of loxP-flanked region: 3.3 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 1 to 5 (including 1333 bp of sequence upstream of exon 1) will result in loss of function of the Gh gene by deleting the complete gene.
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2009.
Gene Family: growth hormone
Genetic Background: C57BL/6
Gene Symbol: Gh
Gene Accession Number: NM_008117.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: growth hormone